Joseph S. Reddy, Laura Heath, Abby Vander Linden, Mariet Allen, Katia de Paiva Lopes, Fatemeh Seifar, Erming Wang, Yiyi Ma, William L. Poehlman, Zachary S. Quicksall, Alexi Runnels, Yanling Wang, Duc M. Duong, Luming Yin, Kaiming Xu, Erica S. Modeste, Anantharaman Shantaraman, Eric B. Dammer, Lingyan Ping, Stephanie R. Oatman, Jo Scanlan, Charlotte Ho, Minerva M. Carrasquillo, Merve Atik, Geovanna Yepez, Adriana O. Mitchell, Thuy T. Nguyen, Xianfeng Chen, David X. Marquez, Hasini Reddy, Harrison Xiao, Sudha Seshadri, Richard Mayeux, Stefan Prokop, Edward B. Lee, Geidy E. Serrano, Thomas G. Beach, Andrew F. Teich, Varham Haroutunian, Edward J. Fox, Marla Gearing, Aliza Wingo, Thomas Wingo, James J. Lah, Allan I. Levey, Dennis W. Dickson, Lisa L. Barnes, Philip De Jager, Bin Zhang, David Bennett, Nicholas T. Seyfried, Anna K. Greenwood, Nilüfer Ertekin-Taner. Bridging the Gap: Multi-Omics Profiling of Brain Tissue in Alzheimer’s Disease and Older Controls in Multi-Ethnic Populations. bioRxiv. 2024:2024.04. 16.589592.
Rowan Saloner, Adam Staffaroni, Eric Dammer, Nicholas Seyfried et al. Large-scale network analysis of the cerebrospinal fluid proteome identifies molecular signatures of frontotemporal lobar degeneration, 28 March 2024, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-4103685/v1]
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Ortlund E, Chen C-Y, Maner-Smith K, Khadka M, Ahn J, Gulbin X, Ivanova A, Dammer E, Seyfried N, Bennett D. Integrative brain omics approach reveals key role for sn-1 lysophosphatidylethanolamine in Alzheimer’s dementia. PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-3973736/v1]
Lizama B, North H, Pandey K, Williams C, Duong D, Cho E, Caro VD, Ping L, Blennow K, Zetterberg H, Lah J, Levey A, Grundman M, Caggiano A, Seyfried N, Hamby M. An interim exploratory biomarker analysis of a Phase 2 clinical trial to assess the impact of CT1812 in Alzheimer’s disease. bioRxiv. 2024:2024.02.16.578765. doi: 10.1101/2024.02.16.578765.
Anantharaman Shantaraman, Eric B. Dammer, Obiadada Ugochukwu, Duc M. Duong, Luming Yin, E. Kathleen Carter, Marla Gearing, Alice Chen-Plotkin, Edward B. Lee, John Q. Trojanowski, David A. Bennett, James J. Lah, Allan I. Levey, Nicholas T. Seyfried, Lenora Higginbotham. Network Proteomics of the Lewy Body Dementia Brain Reveals Presynaptic Signatures Distinct from Alzheimer’s Disease. https://doi.org/10.1101/2024.01.23.576728
Yuanyuan Shen, Muhammad Ali, Menghan Liu, Jigyasha Timsina, Ciyang Wang, Anh Do, Daniel Western, Priyanka Gorijala, John Budde, Haiyan Liu, Brian Gordon, Nelly Joseph-Mathurin, Richard J Perrin, Eric McDade, John C Morris, Jorge J Llibre-Guerra, Randall J Bateman, Dario Maschi, Tony Wyss-Coray, Pau Pastor, Alan E Renton, Ezequiel I Surace, Erik CB Johnson, Ignacio Alvarez, Johannes Levin, John M Ringman, Allan I Levey, Ricardo Francisco Allegri, Nicholas Seyfried, Gregg S Day, Qisi Wu, M Victoria Fernandez, Laura Ibanez, YunJu Sung, Carlos Cruchaga. Systematic proteomics in Autosomal dominant Alzheimer’s disease reveals decades-early changes of CSF proteins in neuronal death, and immune pathways. doi: https://doi.org/10.1101/2024.01.12.24301242
Aleksandra M. Wojtas, Eric B. Dammer, Qi Guo, Lingyan Ping, Ananth Shantaraman, Duc M. Duong, Luming Yin, Edward J. Fox, Fatemeh Seifar, Edward B. Lee, Erik C.B. Johnson, James J. Lah, Allan I. Levey, Yona Levites, Srikant Rangaraju, Todd E. Golde, Nicholas T. Seyfried. Proteomic Changes in the Human Cerebrovasculature in Alzheimer’s Disease and Related Tauopathies Linked to Peripheral Biomarkers in Plasma and Cerebrospinal Fluid. doi: https://doi.org/10.1101/2024.01.10.24301099
Qi Guo, Lingyan Ping,Eric B. Dammer, Duc M. Duong, Luming Yin, Kaiming Xu, Ananth Shantaraman, Edward J. Fox, Erik C.B. Johnson, Blaine R. Roberts, James J. Lah, Allan I. Levey, Nicholas T. Seyfried. Global analysis of the heparin-enriched plasma proteome captures matrisome-associated proteins in Alzheimer’s disease. doi: https://doi.org/10.1101/2023.11.06.565824
Levites Y, Dammer EB, Ran Y, Tsering W, Duong D, Abreha M, Gadhavi J, Lolo K, Trejo-Lopez J, Phillips JL, Iturbe A, Erqiuzi A, Moore BD, Ryu D, Natu A, Dillon KD, Torrellas J, Moran C, Ladd TB, Afroz KF, Islam T, Jagirdar J, Funk CC, Robinson M, Borchelt DR, Ertekin-Taner N, Kelly JW, Heppner FL, Johnson EC, McFarland K, Levey AL, Prokop S, Seyfried NT, Golde TE. Aβ Amyloid Scaffolds the Accumulation of Matrisome and Additional Proteins in Alzheimer’s Disease. bioRxiv. 2023. Epub 2023/12/11. doi: 10.1101/2023.11.29.568318. PubMed PMID: 38076912; PMCID: PMC10705437.
Valborg Gudmundsdottir, Elisabet Frick, Valur Emilsson, Thorarinn Jonmundsson, Anna Steindorsdottir, Erik CB Johnson, Raquel Puerta, Eric Dammer, Anantharaman Shantaraman, Amanda Cano, Merce Boada, Sergi Valero, Pablo Garcia-Gonzalez, Elias Gudmundsson, Alexander Gudjonsson, Rebecca Pitts, Xiazi Qiu, Nancy Finkel, Joseph Loureiro, Anthony Orth, Nicholas Seyfried, Allan Levey, Agustín Ruiz, Thor Aspelund, Lori Jennings, Lenore Launer, Vilmundur Gudnason. Serum proteomics reveals APOE dependent and independent protein signatures in Alzheimer’s disease. https://doi.org/10.21203/rs.3.rs-3706206/v1
Wisch JK, McKay NS, Boerwinkle AH, Kennedy J, Flores S, Handen BL, Christian BT, Head E, Mapstone M, Rafii MS, O’Bryant SE, Price JC, Laymon CM, Krinsky-McHale SJ, Lai F, Rosas HD, Hartley SL, Zaman S, Lott IT, Tudorascu D, Zammit M, Brickman AM, Lee JH, Bird TD, Cohen A, Chrem P, Daniels A, Chhatwal JP, Cruchaga C, Ibanez L, Jucker M, Karch CM, Day GS, Lee JH, Levin J, Llibre-Guerra J, Li Y, Lopera F, Roh JH, Ringman JM, Supnet-Bell C, van Dyck CH, Xiong C, Wang G, Morris JC, McDade E, Bateman RJ, Benzinger TLS, Gordon BA, Ances BM; Alzheimer’s Biomarker Consortium-Down syndrome; Dominantly Inherited Alzheimer Network. Comparison of tau spread in people with Down syndrome versus autosomal-dominant Alzheimer’s disease: a cross-sectional study. Lancet Neurol. 2024 May;23(5):500-510. doi: 10.1016/S1474-4422(24)00084-X. PMID: 38631766.
Wagemann O, Liu H, Wang G, Shi X, Bittner T, Scelsi MA, Farlow MR, Clifford DB, Supnet-Bell C, Santacruz AM, Aschenbrenner AJ, Hassenstab JJ, Benzinger TLS, Gordon BA, Coalier KA, Cruchaga C, Ibanez L, Perrin RJ, Xiong C, Li Y, Morris JC, Lah JJ, Berman SB, Roberson ED, van Dyck CH, Galasko D, Gauthier S, Hsiung G-YR, Brooks WS, Pariente J, Mummery CJ, Day GS, Ringman JM, Mendez PC, St. George-Hyslop P, Fox NC, Suzuki K, Okhravi HR, Chhatwal J, Levin J, Jucker M, Sims JR, Holdridge KC, Proctor NK, Yaari R, Andersen SW, Mancini M, Llibre-Guerra J, Bateman RJ, McDade E, Dominantly Inherited Alzheimer Network–Trials Unit. Downstream Biomarker Effects of Gantenerumab or Solanezumab in Dominantly Inherited Alzheimer Disease: The DIAN-TU-001 Randomized Clinical Trial. JAMA Neurology. 2024. doi: 10.1001/jamaneurol.2024.0991.
Kumar P, Goettemoeller AM, Espinosa-Garcia C, Tobin BR, Tfaily A, Nelson RS, Natu A, Dammer EB, Santiago JV, Malepati S, Cheng L, Xiao H, Duong DD, Seyfried NT, Wood LB, Rowan MJM, Rangaraju S. Native-state proteomics of Parvalbumin interneurons identifies unique molecular signatures and vulnerabilities to early Alzheimer’s pathology. Nature Communications. 2024;15(1):2823. doi: 10.1038/s41467-024-47028-7.
Buchman AS, Yu L, Klein H-U, Zammit AR, Oveisgharan S, Nag S, Tickotsky N, Levy H, Seyfried N, Morgenstern D. Glycoproteome-Wide Discovery of Cortical Glycoproteins That May Provide Cognitive Resilience in Older Adults. Neurology. 2024;102(7):e209223. https://doi.org/10.1212/WNL.0000000000209223
Hsieh YC, Augur ZM, Arbery M, Ashour N, Barrett K, Pearse RV, 2nd, Tio ES, Duong DM, Felsky D, De Jager PL, Bennett DA, Seyfried NT, Young-Pearse TL. Person-specific differences in ubiquitin-proteasome mediated proteostasis in human neurons. Alzheimers Dement. 2024. Epub 2024/03/12. doi: 10.1002/alz.13680. PubMed PMID: 38470006.
Poole VN, Ridwan AR, Arfanakis K, Dawe RJ, Seyfried NT, De Jager PL, Schneider JA, Leurgans SE, Yu L, Bennett DA. Associations of Brain Morphology with Cortical Proteins of Cognitive Resilience. Neurobiology of Aging. 2024. https://doi.org/10.1016/j.neurobiolaging.2024.02.005
Chung M, Carter EK, Veire AM, Dammer EB, Chang J, Duong DM, Raj N, Bassell GJ, Glass JD, Gendron TF, Nelson PT, Levey AI, Seyfried NT, McEachin ZT. Cryptic exon inclusion is a molecular signature of LATE-NC in aging brains. Acta Neuropathologica. 2024;147(1):29. doi: 10.1007/s00401-023-02671-0.
Seddighi S, Qi YA, Brown A-L, Wilkins OG, Bereda C, Belair C, Zhang Y-J, Prudencio M, Keuss MJ, Khandeshi A, Pickles S, Kargbo-Hill SE, Hawrot J, Ramos DM, Yuan H, Roberts J, Sacramento EK, Shah SI, Nalls MA, Colón-Mercado JM, Reyes JF, Ryan VH, Nelson MP, Cook CN, Li Z, Screven L, Kwan JY, Mehta PR, Zanovello M, Hallegger M, Shantaraman A, Ping L, Koike Y, Oskarsson B, Staff NP, Duong DM, Ahmed A, Secrier M, Ule J, Jacobson S, Reich DS, Rohrer JD, Malaspina A, Dickson DW, Glass JD, Ori A, Seyfried NT, Maragkakis M, Petrucelli L, Fratta P, Ward ME. Mis-spliced transcripts generate de novo proteins in TDP-43–related ALS/FTD. Science Translational Medicine. 2024;16(734):eadg7162. doi: doi:10.1126/scitranslmed.adg7162.
Marks JD, Ayuso VE, Carlomagno Y, Yue M, Todd TW, Hao Y, Li Z, McEachin ZT, Shantaraman A, Duong DM, Daughrity LM, Jansen-West K, Shao W, Calliari A, Bejarano JG, DeTure M, Rawlinson B, Casey MC, Lilley MT, Donahue MH, Jawahar VM, Boeve BF, Petersen RC, Knopman DS, Oskarsson B, Graff-Radford NR, Wszolek ZK, Dickson DW, Josephs KA, Qi YA, Seyfried NT, Ward ME, Zhang Y-J, Prudencio M, Petrucelli L, Cook CN. TMEM106B core deposition associates with TDP-43 pathology and is increased in risk SNP carriers for frontotemporal dementia. Science Translational Medicine. 2024;16(730):eadf9735. doi:10.1126/scitranslmed.adf9735.
Wilson KA, Bar S, Dammer EB, Carrera EM, Hodge BA, Hilsabeck TAU, Bons J, Brownridge GW, Beck JN, Rose J, Granath-Panelo M, Nelson CS, Qi G, Gerencser AA, Lan J, Afenjar A, Chawla G, Brem RB, Campeau PM, Bellen HJ, Schilling B, Seyfried NT, Ellerby LM, Kapahi P. OXR1 maintains the retromer to delay brain aging under dietary restriction. Nature Communications. 2024;15(1):467. doi: 10.1038/s41467-023-44343-3.
Zammit AR, Klein H-U, Yu L, Levey AI, Seyfried NT, Wingo AP, Wingo TS, Schneider JA, Bennett DA, Buchman AS. Proteome-wide Analyses Identified Cortical Proteins Associated With Resilience for Varied Cognitive Abilities. Neurology. 2024 Jan 9;102(1):e207816. doi: 10.1212/WNL.0000000000207816. Epub 2023 Dec 15. PMID: 38165375
Wang E, Pan AL, Bagchi P, Rangaraju S, Seyfried NT, Ehrlich ME, Salton SR, Zhang B. Proteomic Signaling of Dual-Specificity Phosphatase 4 (DUSP4) in Alzheimer’s Disease. Biomolecules 2024, 14(1), 66; https://doi.org/10.3390/biom14010066
Chou V, Pearse RV, 2nd, Aylward AJ, Ashour N, Taga M, Terzioglu G, Fujita M, Fancher SB, Sigalov A, Benoit CR, Lee H, Lam M, Seyfried NT, Bennett DA, De Jager PL, Menon V, Young-Pearse TL. INPP5D regulates inflammasome activation in human microglia. Nat Commun. 2023;14(1):7552. Epub 2023/11/29. doi: 10.1038/s41467-023-42819-w. PubMed PMID: 38016942; PMCID: PMC10684891.
Mei Z, Liu J, Schroeder JP, Weinshenker D, Duong DM, Seyfried NT, Li Y, Jin P, Wingo AP, Wingo TS. Lowering Hippocampal miR-29a Expression Slows Cognitive Decline and Reduces Beta-Amyloid Deposition in 5×FAD Mice. Mol Neurobiol. 2023. Epub 2023/11/22. doi: 10.1007/s12035-023-03791-0. PubMed PMID: 37989983.
Santiago JV, Natu A, Ramelow CC, Rayaprolu S, Xiao H, Kumar V, Kumar P, Seyfried NT, Rangaraju S. Identification of State-Specific Proteomic and Transcriptomic Signatures of Microglia-Derived Extracellular Vesicles. Mol Cell Proteomics. 2023;22(12):100678. Epub 2023/11/13. doi: 10.1016/j.mcpro.2023.100678. PubMed PMID: 37952696; PMCID: PMC10755493.
Hurst CD, Dunn AR, Dammer EB, Duong DM, Shapley SM, Seyfried NT, Kaczorowski CC, Johnson ECB. Genetic background influences the 5XFAD Alzheimer’s disease mouse model brain proteome. Front Aging Neurosci. 2023;15:1239116. Epub 2023/10/30. doi: 10.3389/fnagi.2023.1239116. PubMed PMID: 37901791; PMCID: PMC10602695.
Lopes KP, Yu L, Shen X, Qiu Y, Tasaki S, Iatrou A, Beeri MS, Seyfried NT, Menon V, Wang Y, Schneider JA, Cantor H, Bennett DA. Associations of cortical SPP1 and ITGAX with cognition and common neuropathologies in older adults. Alzheimers Dement. 2023. Epub 2023/09/20. doi: 10.1002/alz.13474. PubMed PMID: 37727065.
Higginbotham L, Carter EK, Dammer EB, Haque RU, Johnson ECB, Duong DM, Yin L, De Jager PL, Bennett DA, Felsky D, Tio ES, Lah JJ, Levey AI, Seyfried NT. Unbiased classification of the elderly human brain proteome resolves distinct clinical and pathophysiological subtypes of cognitive impairment. Neurobiol Dis. 2023;186:106286. Epub 2023/09/10. doi: 10.1016/j.nbd.2023.106286. PubMed PMID: 37689213; PMCID: PMC10750427.
Haque R, Watson CM, Liu J, Carter EK, Duong DM, Lah JJ, Wingo AP, Roberts BR, Johnson ECB, Saykin AJ, Shaw LM, Seyfried NT, Wingo TS, Levey AI. A protein panel in cerebrospinal fluid for diagnostic and predictive assessment of Alzheimer’s disease. Sci Transl Med. 2023;15(712):eadg4122. Epub 2023/09/06. doi: 10.1126/scitranslmed.adg4122. PubMed PMID: 37672565.
Wingo AP, Liu Y, Gerasimov ES, Vattathil SM, Liu J, Cutler DJ, Epstein MP, Blokland GAM, Thambisetty M, Troncoso JC, Duong DM, Bennett DA, Levey AI, Seyfried NT, Wingo TS. Sex differences in brain protein expression and disease. Nat Med. 2023;29(9):2224-32. Epub 2023/09/01. doi: 10.1038/s41591-023-02509-y. PubMed PMID: 37653343; PMCID: PMC10504083.
Sukoff Rizzo SJ, Homanics G, Schaeffer DJ, Schaeffer L, Park JE, Oluoch J, Zhang T, Haber A, Seyfried NT, Paten B, Greenwood A, Murai T, Choi SH, Huhe H, Kofler J, Strick PL, Carter GW, Silva AC. Bridging the rodent to human translational gap: Marmosets as model systems for the study of Alzheimer’s disease. Alzheimers Dement (N Y). 2023;9(3):e12417. Epub 2023/08/24. doi: 10.1002/trc2.12417. PubMed PMID: 37614242; PMCID: PMC10442521.
Lee H, Aylward AJ, Pearse RV, 2nd, Lish AM, Hsieh YC, Augur ZM, Benoit CR, Chou V, Knupp A, Pan C, Goberdhan S, Duong DM, Seyfried NT, Bennett DA, Taga MF, Huynh K, Arnold M, Meikle PJ, De Jager PL, Menon V, Young JE, Young-Pearse TL. Cell-type-specific regulation of APOE and CLU levels in human neurons by the Alzheimer’s disease risk gene SORL1. Cell Rep. 2023;42(8):112994. Epub 2023/08/24. doi: 10.1016/j.celrep.2023.112994. PubMed PMID: 37611586; PMCID: PMC10568487.
Borkowski K, Seyfried NT, Arnold M, Lah JJ, Levey AI, Hales CM, Dammer EB, Blach C, Louie G, Kaddurah-Daouk R, Newman JW. Integration of plasma and CSF metabolomics with CSF proteomic reveals novel associations between lipid mediators and central nervous system vascular and energy metabolism. Sci Rep. 2023;13(1):13752. Epub 2023/08/24. doi: 10.1038/s41598-023-39737-8.
Johnson ECB, Bian S, Haque RU, Carter EK, Watson CM, Gordon BA, Ping L, Duong DM, Epstein MP, McDade E, Barthélemy NR, Karch CM, Xiong C, Cruchaga C, Perrin RJ, Wingo AP, Wingo TS, Chhatwal JP, Day GS, Noble JM, Berman SB, Martins R, Graff-Radford NR, Schofield PR, Ikeuchi T, Mori H, Levin J, Farlow M, Lah JJ, Haass C, Jucker M, Morris JC, Benzinger TLS, Roberts BR, Bateman RJ, Fagan AM, Seyfried NT, Levey AI. Cerebrospinal fluid proteomics define the natural history of autosomal dominant Alzheimer’s disease. Nat Med. 2023;29(8):1979-88. Epub 2023/08/08. doi: 10.1038/s41591-023-02476-4. PubMed PMID: 37550416; PMCID: PMC10427428 revXon.
Modeste ES, Ping L, Watson CM, Duong DM, Dammer EB, Johnson ECB, Roberts BR, Lah JJ, Levey AI, Seyfried NT. Quantitative proteomics of cerebrospinal fluid from African Americans and Caucasians reveals shared and divergent changes in Alzheimer’s disease. Mol Neurodegener. 2023;18(1):48. Epub 2023/07/20. doi: 10.1186/s13024-023-00638-z. PubMed PMID: 37468915; PMCID: PMC10355042.
Sung YJ, Yang C, Norton J, Johnson M, Fagan A, Bateman RJ, Perrin RJ, Morris JC, Farlow MR, Chhatwal JP, Schofield PR, Chui H, Wang F, Novotny B, Eteleeb A, Karch C, Schindler SE, Rhinn H, Johnson ECB, Oh HS, Rutledge JE, Dammer EB, Seyfried NT, Wyss-Coray T, Harari O, Cruchaga C. Proteomics of brain, CSF, and plasma identifies molecular signatures for distinguishing sporadic and genetic Alzheimer’s disease. Sci Transl Med. 2023;15(703):eabq5923. Epub 2023/07/05. doi: 10.1126/scitranslmed.abq5923. PubMed PMID: 37406134.
Head PE, Kapoor-Vazirani P, Nagaraju GP, Zhang H, Rath SK, Luong NC, Haji-Seyed-Javadi R, Sesay F, Wang SY, Duong DM, Daddacha W, Minten EV, Song B, Danelia D, Liu X, Li S, Ortlund EA, Seyfried NT, Smalley DM, Wang Y, Deng X, Dynan WS, El-Rayes B, Davis AJ, Yu DS. DNA-PK is activated by SIRT2 deacetylation to promote DNA double-strand break repair by non-homologous end joining. Nucleic Acids Res. 2023;51(15):7972-87. Epub 2023/07/03. doi: 10.1093/nar/gkad549. PubMed PMID: 37395399; PMCID: PMC10450170.
Seto M, Dumitrescu L, Mahoney ER, Sclafani AM, De Jager PL, Menon V, Koran MEI, Robinson RA, Ruderfer DM, Cox NJ, Seyfried NT, Jefferson AL, Schneider JA, Bennett DA, Petyuk VA, Hohman TJ. Multi-omic characterization of brain changes in the vascular endothelial growth factor family during aging and Alzheimer’s disease. Neurobiol Aging. 2023;126:25-33. Epub 2023/03/12. doi: 10.1016/j.neurobiolaging.2023.01.010. PubMed PMID: 36905877; PMCID: PMC10106439.
Yu M, Ye H, De-Paula RB, Mangleburg CG, Wu T, Lee TV, Li Y, Duong D, Phillips B, Cruchaga C, Allen GI, Seyfried NT, Al-Ramahi I, Botas J, Shulman JM. Functional screening of lysosomal storage disorder genes identifies modifiers of alpha-synuclein neurotoxicity. PLoS Genet. 2023;19(5):e1010760. Epub 2023/05/18. doi: 10.1371/journal.pgen.1010760. PubMed PMID: 37200393; PMCID: PMC10231792.
Walker CK, Greathouse KM, Tuscher JJ, Dammer EB, Weber AJ, Liu E, Curtis KA, Boros BD, Freeman CD, Seo JV, Ramdas R, Hurst C, Duong DM, Gearing M, Murchison CF, Day JJ, Seyfried NT, Herskowitz JH. Cross-Platform Synaptic Network Analysis of Human Entorhinal Cortex Identifies TWF2 as a Modulator of Dendritic Spine Length. J Neurosci. 2023;43(20):3764-85. Epub 2023/04/14. doi: 10.1523/jneurosci.2102-22.2023. PubMed PMID: 37055180; PMCID: PMC10198456.
Wynne ME, Ogunbona O, Lane AR, Gokhale A, Zlatic SA, Xu C, Wen Z, Duong DM, Rayaprolu S, Ivanova A, Ortlund EA, Dammer EB, Seyfried NT, Roberts BR, Crocker A, Shanbhag V, Petris M, Senoo N, Kandasamy S, Claypool SM, Barrientos A, Wingo A, Wingo TS, Rangaraju S, Levey AI, Werner E, Faundez V. APOE expression and secretion are modulated by mitochondrial dysfunction. Elife. 2023;12. Epub 2023/05/12. doi: 10.7554/eLife.85779. PubMed PMID: 37171075; PMCID: PMC10231934.
Watson CM, Dammer EB, Ping L, Duong DM, Modeste E, Carter EK, Johnson ECB, Levey AI, Lah JJ, Roberts BR, Seyfried NT. Quantitative Mass Spectrometry Analysis of Cerebrospinal Fluid Protein Biomarkers in Alzheimer’s Disease. Sci Data. 2023;10(1):261. Epub 2023/05/10. doi: 10.1038/s41597-023-02158-3. PubMed PMID: 37160957; PMCID: PMC10170100.
Dammer EB, Seyfried NT, Johnson ECB. Batch Correction and Harmonization of -Omics Datasets with a Tunable Median Polish of Ratio. Front Syst Biol. 2023;3. Epub 2023/05/01. doi: 10.3389/fsysb.2023.1092341. PubMed PMID: 37122388; PMCID: PMC10137904.
Harbin NH, Lustberg DJ, Hurst C, Pare JF, Crotty KM, Waters AL, Yeligar SM, Smith Y, Seyfried NT, Weinshenker D, Hepler JR. RGS14 limits seizure-induced mitochondrial oxidative stress and pathology in hippocampus. Neurobiol Dis. 2023 Jun 1:181:106128. Epub 2023/04/17. doi: 10.1016/j.nbd.2023.106128.
Sunna S, Bowen C, Zeng H, Rayaprolu S, Kumar P, Bagchi P, Dammer EB, Guo Q, Duong DM, Bitarafan S, Natu A, Wood L, Seyfried NT, Rangaraju S. Cellular Proteomic Profiling Using Proximity Labeling by TurboID-NES in Microglial and Neuronal Cell Lines. Mol Cell Proteomics. 2023;22(6):100546. Epub 2023/04/16. doi: 10.1016/j.mcpro.2023.100546. PubMed PMID: 37061046; PMCID: PMC10205547.
Hurst C, Pugh DA, Abreha MH, Duong DM, Dammer EB, Bennett DA, Herskowitz JH, Seyfried NT. Integrated Proteomics to Understand the Role of Neuritin (NRN1) as a Mediator of Cognitive Resilience to Alzheimer’s Disease. Mol Cell Proteomics. 2023;22(5):100542. Epub 2023/04/07. doi: 10.1016/j.mcpro.2023.100542. PubMed PMID: 37024090; PMCID: PMC10233303.
Buchman AS, Yu L, Klein HU, Zammit AR, Oveisgharan S, Grodstein F, Tasaki S, Levey AI, Seyfried NT, Bennett DA. Proteome-Wide Discovery of Cortical Proteins That May Provide Motor Resilience to Offset the Negative Effects of Pathologies in Older Adults. J Gerontol A Biol Sci Med Sci. 2023;78(3):494-503. Epub 2022/05/06. doi: 10.1093/gerona/glac105.
Čarna M, Onyango IG, Katina S, Holub D, Novotny JS, Nezvedova M, Jha D, Nedelska Z, Lacovich V, Vyvere TV, Houbrechts R, Garcia-Mansfield K, Sharma R, David-Dirgo V, Vyhnalek M, Texlova K, Chaves H, Bakkar N, Pertierra L, Vinkler M, Markova H, Laczo J, Sheardova K, Hortova-Kohoutkova M, Frič J, Forte G, Kaňovsky P, Belaškova S, Damborsky J, Hort J, Seyfried NT, Bowser R, Sevlever G, Rissman RA, Smith RA, Hajduch M, Pirrotte P, Spacil Z, Dammer EB, Limbäck-Stokin C, Stokin GB. Pathogenesis of Alzheimer’s disease: Involvement of the choroid plexus. Alzheimers Dement. 2023;19(8):3537-54. Epub 2023/02/25. doi: 10.1002/alz.12970.
Tandon R, Levey AI, Lah JJ, Seyfried NT, Mitchell CS. Machine Learning Selection of Most Predictive Brain Proteins Suggests Role of Sugar Metabolism in Alzheimer’s Disease. J Alzheimers Dis. 2023;92(2):411-24. Epub 2023/02/14. doi: 10.3233/jad-220683. PubMed PMID: 36776048; PMCID: PMC10041447.
Dammer EB, Ping L, Duong DM, Modeste ES, Seyfried NT, Lah JJ, Levey AI, Johnson ECB. Multi-platform proteomic analysis of Alzheimer’s disease cerebrospinal fluid and plasma reveals network biomarkers associated with proteostasis and the matrisome. Alzheimers Res Ther. 2022;14(1):174. Epub 2022/11/18. doi: 10.1186/s13195-022-01113-5. PubMed PMID: 36384809; PMCID: PMC9670630.
Sohn AL, Ping L, Glass JD, Seyfried NT, Hector EC, Muddiman DC. Interrogating the Metabolomic Profile of Amyotrophic Lateral Sclerosis in the Post-Mortem Human Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Mass Spectrometry Imaging (MSI). Metabolites. 2022;12(11). Epub 2022/11/11. doi: 10.3390/metabo12111096. PubMed PMID: 36355179; PMCID: PMC9696666.
Del Campo M, Peeters CFW, Johnson ECB, Vermunt L, Hok AHYS, van Nee M, Chen-Plotkin A, Irwin DJ, Hu WT, Lah JJ, Seyfried NT, Dammer EB, Herradon G, Meeter LH, van Swieten J, Alcolea D, Lleó A, Levey AI, Lemstra AW, Pijnenburg YAL, Visser PJ, Tijms BM, van der Flier WM, Teunissen CE. CSF proteome profiling across the Alzheimer’s disease spectrum reflects the multifactorial nature of the disease and identifies specific biomarker panels. Nat Aging. 2022;2(11):1040-53. Epub 2023/04/29. doi: 10.1038/s43587-022-00300-1. PubMed PMID: 37118088; PMCID: PMC10292920.
Kapoor-Vazirani P, Rath SK, Liu X, Shu Z, Bowen NE, Chen Y, Haji-Seyed-Javadi R, Daddacha W, Minten EV, Danelia D, Farchi D, Duong DM, Seyfried NT, Deng X, Ortlund EA, Kim B, Yu DS. SAMHD1 deacetylation by SIRT1 promotes DNA end resection by facilitating DNA binding at double-strand breaks. Nat Commun. 2022;13(1):6707. Epub 2022/11/08. doi: 10.1038/s41467-022-34578-x. PubMed PMID: 36344525; PMCID: PMC9640623.
Suttapitugsakul S, Stavenhagen K, Donskaya S, Bennett DA, Mealer RG, Seyfried NT, Cummings RD. Glycoproteomics Landscape of Asymptomatic and Symptomatic Human Alzheimer’s Disease Brain. Mol Cell Proteomics. 2022;21(12):100433. Epub 2022/10/30. doi: 10.1016/j.mcpro.2022.100433. PubMed PMID: 36309312; PMCID: PMC9706167.
Hsieh YC, Negri J, He A, Pearse RV, 2nd, Liu L, Duong DM, Chibnik LB, Bennett DA, Seyfried NT, Young-Pearse TL. Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity. Mol Neurodegener. 2022;17(1):61. Epub 2022/09/22. doi: 10.1186/s13024-022-00558-4. PubMed PMID: 36131294; PMCID: PMC9494921.
Yu L, Hsieh YC, Pearse RV, Wang Y, Petyuk VA, Schneider JA, Buchman AS, Seyfried NT, De Jager PL, Young-Pearse TL, Bennett DA. Association of AK4 Protein From Stem Cell-Derived Neurons With Cognitive Reserve: An Autopsy Study. Neurology. 2022;99(20):e2264-e74. Epub 2022/08/11. doi: 10.1212/wnl.0000000000201120. PubMed PMID: 35948448; PMCID: PMC9694839.
Wingo TS, Liu Y, Gerasimov ES, Vattathil SM, Wynne ME, Liu J, Lori A, Faundez V, Bennett DA, Seyfried NT, Levey AI, Wingo AP. Shared mechanisms across the major psychiatric and neurodegenerative diseases. Nat Commun. 2022;13(1):4314. Epub 2022/07/27. doi: 10.1038/s41467-022-31873-5. PubMed PMID: 35882878; PMCID: PMC9325708.
Batra R, Arnold M, Wörheide MA, Allen M, Wang X, Blach C, Levey AI, Seyfried NT, Ertekin-Taner N, Bennett DA, Kastenmüller G, Kaddurah-Daouk RF, Krumsiek J. The landscape of metabolic brain alterations in Alzheimer’s disease. Alzheimers Dement. 2022. Epub 2022/07/14. doi: 10.1002/alz.12714. PubMed PMID: 35829654; PMCID: PMC9837312.
Nelson RS, Dammer EB, Santiago JV, Seyfried NT, Rangaraju S. Brain Cell Type-Specific Nuclear Proteomics Is Imperative to Resolve Neurodegenerative Disease Mechanisms. Front Neurosci. 2022;16:902146. Epub 2022/07/06. doi: 10.3389/fnins.2022.902146. PubMed PMID: 35784845; PMCID: PMC9243337.
Olah VJ, Goettemoeller AM, Rayaprolu S, Dammer EB, Seyfried NT, Rangaraju S, Dimidschstein J, Rowan MJM. Biophysical K(v)3 channel alterations dampen excitability of cortical PV interneurons and contribute to network hyperexcitability in early Alzheimer’s. Elife. 2022;11. Epub 2022/06/22. doi: 10.7554/eLife.75316. PubMed PMID: 35727131; PMCID: PMC9278953.
Johnson MA, Nuckols TA, Merino P, Bagchi P, Nandy S, Root J, Taylor G, Seyfried NT, Kukar T. Proximity-based labeling reveals DNA damage-induced phosphorylation of fused in sarcoma (FUS) causes distinct changes in the FUS protein interactome. J Biol Chem. 2022;298(8):102135. Epub 2022/06/17. doi: 10.1016/j.jbc.2022.102135. PubMed PMID: 35709984; PMCID: PMC9372748.
Eninger T, Müller SA, Bacioglu M, Schweighauser M, Lambert M, Maia LF, Neher JJ, Hornfeck SM, Obermüller U, Kleinberger G, Haass C, Kahle PJ, Staufenbiel M, Ping L, Duong DM, Levey AI, Seyfried NT, Lichtenthaler SF, Jucker M, Kaeser SA. Signatures of glial activity can be detected in the CSF proteome. Proc Natl Acad Sci U S A. 2022;119(24):e2119804119. Epub 2022/06/07. doi: 10.1073/pnas.2119804119. PubMed PMID: 35666874; PMCID: PMC9214531.
Levey AI, Qiu D, Zhao L, Hu WT, Duong DM, Higginbotham L, Dammer EB, Seyfried NT, Wingo TS, Hales CM, Gámez Tansey M, Goldstein DS, Abrol A, Calhoun VD, Goldstein FC, Hajjar I, Fagan AM, Galasko D, Edland SD, Hanfelt J, Lah JJ, Weinshenker D. A phase II study repurposing atomoxetine for neuroprotection in mild cognitive impairment. Brain. 2022;145(6):1924-38. Epub 2021/12/18. doi: 10.1093/brain/awab452. PubMed PMID: 34919634; PMCID: PMC9630662.
Rayaprolu S, Bitarafan S, Santiago JV, Betarbet R, Sunna S, Cheng L, Xiao H, Nelson RS, Kumar P, Bagchi P, Duong DM, Goettemoeller AM, Oláh VJ, Rowan M, Levey AI, Wood LB, Seyfried NT, Rangaraju S. Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain. Nat Commun. 2022;13(1):2927. Epub 2022/05/26. doi: 10.1038/s41467-022-30623-x. PubMed PMID: 35614064; PMCID: PMC9132937.
Trumpff C, Owusu-Ansah E, Klein HU, Lee AJ, Petyuk V, Wingo TS, Wingo AP, Thambisetty M, Ferrucci L, Seyfried NT, Bennett DA, De Jager PL, Picard M. Mitochondrial respiratory chain protein co-regulation in the human brain. Heliyon. 2022;8(5):e09353. Epub 2022/05/24. doi: 10.1016/j.heliyon.2022.e09353. PubMed PMID: 35600441; PMCID: PMC9118667.
Wingo TS, Gerasimov ES, Liu Y, Duong DM, Vattathil SM, Lori A, Gockley J, Breen MS, Maihofer AX, Nievergelt CM, Koenen KC, Levey DF, Gelernter J, Stein MB, Ressler KJ, Bennett DA, Levey AI, Seyfried NT, Wingo AP. Integrating human brain proteomes with genome-wide association data implicates novel proteins in post-traumatic stress disorder. Mol Psychiatry. 2022;27(7):3075-84. Epub 2022/04/23. doi: 10.1038/s41380-022-01544-4. PubMed PMID: 35449297; PMCID: PMC9233006.
Zhang H, Dammer EB, Duong DM, Danelia D, Seyfried NT, Yu DS. Quantitative proteomic analysis of the lysine acetylome reveals diverse SIRT2 substrates. Sci Rep. 2022;12(1):3822. Epub 2022/03/11. doi: 10.1038/s41598-022-06793-5. PubMed PMID: 35264593; PMCID: PMC8907344.
Yu L, Boyle PA, Wingo AP, Yang J, Wang T, Buchman AS, Wingo TS, Seyfried NT, Levey AI, De Jager PL, Schneider JA, Bennett DA. Neuropathologic Correlates of Human Cortical Proteins in Alzheimer Disease and Related Dementias. Neurology. 2022;98(10):e1031-e9. Epub 2021/12/24. doi: 10.1212/wnl.0000000000013252. PubMed PMID: 34937778; PMCID: PMC8967389.
Wu CI, Vinton EA, Pearse RV, 2nd, Heo K, Aylward AJ, Hsieh YC, Bi Y, Adeleye S, Fancher S, Duong DM, Seyfried NT, Schwarz TL, Young-Pearse TL. APP and DYRK1A regulate axonal and synaptic vesicle protein networks and mediate Alzheimer’s pathology in trisomy 21 neurons. Mol Psychiatry. 2022;27(4):1970-89. Epub 2022/02/24. doi: 10.1038/s41380-022-01454-5. PubMed PMID: 35194165; PMCID: PMC9133025.
Harerimana NV, Liu Y, Gerasimov ES, Duong D, Beach TG, Reiman EM, Schneider JA, Boyle P, Lori A, Bennett DA, Lah JJ, Levey AI, Seyfried NT, Wingo TS, Wingo AP. Genetic Evidence Supporting a Causal Role of Depression in Alzheimer’s Disease. Biol Psychiatry. 2022;92(1):25-33. Epub 2022/02/19. doi: 10.1016/j.biopsych.2021.11.025. PubMed PMID: 35177243; PMCID: PMC9200901.
Johnson ECB, Carter EK, Dammer EB, Duong DM, Gerasimov ES, Liu Y, Liu J, Betarbet R, Ping L, Yin L, Serrano GE, Beach TG, Peng J, De Jager PL, Haroutunian V, Zhang B, Gaiteri C, Bennett DA, Gearing M, Wingo TS, Wingo AP, Lah JJ, Levey AI, Seyfried NT. Large-scale deep multi-layer analysis of Alzheimer’s disease brain reveals strong proteomic disease-related changes not observed at the RNA level. Nat Neurosci. 2022;25(2):213-25. Epub 2022/02/05. doi: 10.1038/s41593-021-00999-y. PubMed PMID: 35115731; PMCID: PMC8825285.
Wingo AP, Wang M, Liu J, Breen MS, Yang HS, Tang B, Schneider JA, Seyfried NT, Lah JJ, Levey AI, Bennett DA, Jin P, De Jager PL, Wingo TS. Brain microRNAs are associated with variation in cognitive trajectory in advanced age. Transl Psychiatry. 2022;12(1):47. Epub 2022/02/03. doi: 10.1038/s41398-022-01806-3. PubMed PMID: 35105862; PMCID: PMC8807720.
Wang X, Allen M, İş Ö, Reddy JS, Tutor-New FQ, Castanedes Casey M, Carrasquillo MM, Oatman SR, Min Y, Asmann YW, Funk C, Nguyen T, Ho CC, Malphrus KG, Seyfried NT, Levey AI, Younkin SG, Murray ME, Dickson DW, Price ND, Golde TE, Ertekin-Taner N. Alzheimer’s disease and progressive supranuclear palsy share similar transcriptomic changes in distinct brain regions. J Clin Invest. 2022;132(2). Epub 2021/11/24. doi: 10.1172/jci149904. PubMed PMID: 34813500; PMCID: PMC8759790.
Grodstein F, Yu L, de Jager PL, Levey A, Seyfried NT, Bennett DA. Exploring Cortical Proteins Underlying the Relation of Neuroticism to Cognitive Resilience. Aging Brain. 2022;2. Epub 2022/01/01. doi: 10.1016/j.nbas.2022.100031. PubMed PMID: 36874358; PMCID: PMC9979250.
Arakhamia T, Lee CE, Carlomagno Y, Kumar M, Duong DM, Wesseling H, Kundinger SR, Wang K, Williams D, DeTure M, Dickson DW, Cook CN, Seyfried NT, Petrucelli L, Steen JA, Fitzpatrick AWP. Posttranslational Modifications Mediate the Structural Diversity of Tauopathy Strains. Cell. 2021;184(25):6207-10. Epub 2021/12/11. doi: 10.1016/j.cell.2021.11.029. PubMed PMID: 34890553; PMCID: PMC9013541.
Haytural H, Benfeitas R, Schedin-Weiss S, Bereczki E, Rezeli M, Unwin RD, Wang X, Dammer EB, Johnson ECB, Seyfried NT, Winblad B, Tijms BM, Visser PJ, Frykman S, Tjernberg LO. Insights into the changes in the proteome of Alzheimer disease elucidated by a meta-analysis. Sci Data. 2021;8(1):312. Epub 2021/12/05. doi: 10.1038/s41597-021-01090-8. PubMed PMID: 34862388; PMCID: PMC8642431.
Ma Y, Dammer EB, Felsky D, Duong DM, Klein HU, White CC, Zhou M, Logsdon BA, McCabe C, Xu J, Wang M, Wingo TS, Lah JJ, Zhang B, Schneider J, Allen M, Wang X, Ertekin-Taner N, Seyfried NT, Levey AI, Bennett DA, De Jager PL. Atlas of RNA editing events affecting protein expression in aged and Alzheimer’s disease human brain tissue. Nat Commun. 2021;12(1):7035. Epub 2021/12/04. doi: 10.1038/s41467-021-27204-9. PubMed PMID: 34857756; PMCID: PMC8640037.
Wang X, Allen M, İş Ö, Reddy JS, Tutor-New FQ, Castanedes Casey M, Carrasquillo MM, Oatman SR, Min Y, Asmann YW, Funk C, Nguyen T, Ho CC, Malphrus KG, Seyfried NT, Levey AI, Younkin SG, Murray ME, Dickson DW, Price ND, Golde TE, Ertekin-Taner N. Alzheimer’s disease and progressive supranuclear palsy share similar transcriptomic changes in distinct brain regions. J Clin Invest. 2022 Jan 18;132(2):e149904. doi: 10.1172/JCI149904.
Wang Y, Liu R, Liao J, Jiang L, Jeong GH, Zhou L, Polite M, Duong D, Seyfried NT, Wang H, Kiyokawa H, Yin J. Orthogonal ubiquitin transfer reveals human papillomavirus E6 downregulates nuclear transport to disarm interferon-γ dependent apoptosis of cervical cancer cells. Faseb j. 2021;35(11):e21986. Epub 2021/10/19. doi: 10.1096/fj.202101232RR. PubMed PMID: 34662469.
Gorkhali R, Tian L, Dong B, Bagchi P, Deng X, Pawar S, Duong D, Fang N, Seyfried NT, Yang J. Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway. Sci Rep. 2021 Oct 18;11(1):20576. doi: 10.1038/s41598-021-00067-2.
Kundinger SR, Dammer EB, Yin L, Hurst C, Shapley S, Ping L, Khoshnevis S, Ghalei H, Duong DM, Seyfried NT. Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization. J Biol Chem. 2021;297(5):101306. Epub 2021/10/22. doi: 10.1016/j.jbc.2021.101306. PubMed PMID: 34673031; PMCID: PMC8569591.
Borkowski K, Pedersen TL, Seyfried NT, Lah JJ, Levey AI, Hales CM, Dammer EB, Blach C, Louie G, Kaddurah-Daouk R, Newman JW. Association of plasma and CSF cytochrome P450, soluble epoxide hydrolase, and ethanolamide metabolism with Alzheimer’s disease. Alzheimers Res Ther. 2021;13(1):149. Epub 2021/09/08. doi: 10.1186/s13195-021-00893-6. PubMed PMID: 34488866; PMCID: PMC8422756.
Lagomarsino VN, Pearse RV, 2nd, Liu L, Hsieh YC, Fernandez MA, Vinton EA, Paull D, Felsky D, Tasaki S, Gaiteri C, Vardarajan B, Lee H, Muratore CR, Benoit CR, Chou V, Fancher SB, He A, Merchant JP, Duong DM, Martinez H, Zhou M, Bah F, Vicent MA, Stricker JMS, Xu J, Dammer EB, Levey AI, Chibnik LB, Menon V, Seyfried NT, De Jager PL, Noggle S, Selkoe DJ, Bennett DA, Young-Pearse TL. Stem cell-derived neurons reflect features of protein networks, neuropathology, and cognitive outcome of their aged human donors. Neuron. 2021;109(21):3402-20.e9. Epub 2021/09/03. doi: 10.1016/j.neuron.2021.08.003. PubMed PMID: 34473944; PMCID: PMC8571042.
Hampel H, Nisticò R, Seyfried NT, Levey AI, Modeste E, Lemercier P, Baldacci F, Toschi N, Garaci F, Perry G, Emanuele E, Valenzuela PL, Lucia A, Urbani A, Sancesario GM, Mapstone M, Corbo M, Vergallo A, Lista S. Omics sciences for systems biology in Alzheimer’s disease: State-of-the-art of the evidence. Ageing Res Rev. 2021;69:101346. Epub 2021/04/30. doi: 10.1016/j.arr.2021.101346. PubMed PMID: 33915266.
Gokhale A, Lee CE, Zlatic SA, Freeman AAH, Shearing N, Hartwig C, Ogunbona O, Bassell JL, Wynne ME, Werner E, Xu C, Wen Z, Duong D, Seyfried NT, Bearden CE, Oláh VJ, Rowan MJM, Glausier JR, Lewis DA, Faundez V. Mitochondrial Proteostasis Requires Genes Encoded in a Neurodevelopmental Syndrome Locus. J Neurosci. 2021;41(31):6596-616. Epub 2021/07/16. doi: 10.1523/jneurosci.2197-20.2021. PubMed PMID: 34261699; PMCID: PMC8336702.
Peoples JN, Ghazal N, Duong DM, Hardin KR, Manning JR, Seyfried NT, Faundez V, Kwong JQ. Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome. Am J Physiol Cell Physiol. 2021;321(3):C519-c34. Epub 2021/07/29. doi: 10.1152/ajpcell.00156.2021. PubMed PMID: 34319827; PMCID: PMC8461815.
Wingo TS, Liu Y, Gerasimov ES, Gockley J, Logsdon BA, Duong DM, Dammer EB, Lori A, Kim PJ, Ressler KJ, Beach TG, Reiman EM, Epstein MP, De Jager PL, Lah JJ, Bennett DA, Seyfried NT, Levey AI, Wingo AP. Brain proteome-wide association study implicates novel proteins in depression pathogenesis. Nat Neurosci. 2021;24(6):810-7. Epub 2021/04/14. doi: 10.1038/s41593-021-00832-6. PubMed PMID: 33846625; PMCID: PMC8530461.
Gutierrez-Quiceno L, Dammer EB, Johnson AG, Webster JA, Shah R, Duong D, Yin L, Seyfried NT, Alvarez VE, Stein TD, McKee AC, Hales CM. A proteomic network approach resolves stage-specific molecular phenotypes in chronic traumatic encephalopathy. Mol Neurodegener. 2021;16(1):40. Epub 2021/06/27. doi: 10.1186/s13024-021-00462-3. PubMed PMID: 34172091; PMCID: PMC8235576.
Vattathil SM, Liu Y, Harerimana NV, Lori A, Gerasimov ES, Beach TG, Reiman EM, De Jager PL, Schneider JA, Bennett DA, Seyfried NT, Levey AI, Wingo AP, Wingo TS. A Genetic Study of Cerebral Atherosclerosis Reveals Novel Associations with NTNG1 and CNOT3. Genes (Basel). 2021;12(6). Epub 2021/06/03. doi: 10.3390/genes12060815. PubMed PMID: 34073619; PMCID: PMC8228534.
Abreha MH, Ojelade S, Dammer EB, McEachin ZT, Duong DM, Gearing M, Bassell GJ, Lah JJ, Levey AI, Shulman JM, Seyfried NT. TBK1 interacts with tau and enhances neurodegeneration in tauopathy. J Biol Chem. 2021;296:100760. Epub 2021/05/10. doi: 10.1016/j.jbc.2021.100760. PubMed PMID: 33965374; PMCID: PMC8191334.
Dill CD, Dammer EB, Griffen TL, Seyfried NT, Lillard JW, Jr. A network approach reveals driver genes associated with survival of patients with triple-negative breast cancer. iScience. 2021;24(5):102451. Epub 2021/05/20. doi: 10.1016/j.isci.2021.102451. PubMed PMID: 34007962; PMCID: PMC8111681.
Guo Q, Dammer EB, Zhou M, Kundinger SR, Gearing M, Lah JJ, Levey AI, Shulman JM, Seyfried NT. Targeted Quantification of Detergent-Insoluble RNA-Binding Proteins in Human Brain Reveals Stage and Disease Specific Co-aggregation in Alzheimer’s Disease. Front Mol Neurosci. 2021;14:623659. Epub 2021/04/06. doi: 10.3389/fnmol.2021.623659. PubMed PMID: 33815056; PMCID: PMC8014091.
Chen MJ, Ramesha S, Weinstock LD, Gao T, Ping L, Xiao H, Dammer EB, Duong DD, Levey AI, Lah JJ, Seyfried NT, Wood LB, Rangaraju S. Extracellular signal-regulated kinase regulates microglial immune responses in Alzheimer’s disease. J Neurosci Res. 2021;99(6):1704-21. Epub 2021/03/18. doi: 10.1002/jnr.24829. PubMed PMID: 33729626; PMCID: PMC8919593.
Ramesha S, Rayaprolu S, Bowen CA, Giver CR, Bitarafan S, Nguyen HM, Gao T, Chen MJ, Nwabueze N, Dammer EB, Engstrom AK, Xiao H, Pennati A, Seyfried NT, Katz DJ, Galipeau J, Wulff H, Waller EK, Wood LB, Levey AI, Rangaraju S. Unique molecular characteristics and microglial origin of Kv1.3 channel-positive brain myeloid cells in Alzheimer’s disease. Proc Natl Acad Sci U S A. 2021;118(11). Epub 2021/03/03. doi: 10.1073/pnas.2013545118. PubMed PMID: 33649184; PMCID: PMC7980378.
Robins C, Liu Y, Fan W, Duong DM, Meigs J, Harerimana NV, Gerasimov ES, Dammer EB, Cutler DJ, Beach TG, Reiman EM, De Jager PL, Bennett DA, Lah JJ, Wingo AP, Levey AI, Seyfried NT, Wingo TS. Genetic control of the human brain proteome. Am J Hum Genet. 2021;108(3):400-10. Epub 2021/02/12. doi: 10.1016/j.ajhg.2021.01.012. PubMed PMID: 33571421; PMCID: PMC8008492.
Wingo AP, Liu Y, Gerasimov ES, Gockley J, Logsdon BA, Duong DM, Dammer EB, Robins C, Beach TG, Reiman EM, Epstein MP, De Jager PL, Lah JJ, Bennett DA, Seyfried NT, Levey AI, Wingo TS. Integrating human brain proteomes with genome-wide association data implicates new proteins in Alzheimer’s disease pathogenesis. Nat Genet. 2021;53(2):143-6. Epub 2021/01/30. doi: 10.1038/s41588-020-00773-z. PubMed PMID: 33510477; PMCID: PMC8130821.
Koren SA, Hamm MJ, Cloyd R, Fontaine SN, Chishti E, Lanzillotta C, Rodriguez-Rivera J, Ingram A, Bell M, Galvis-Escobar SM, Zulia N, Di Domenico F, Duong D, Seyfried NT, Powell D, Vandsburger M, Frolinger T, Hartz AMS, Koren J, 3rd, Axten JM, Laping NJ, Abisambra JF. Broad Kinase Inhibition Mitigates Early Neuronal Dysfunction in Tauopathy. Int J Mol Sci. 2021;22(3). Epub 2021/02/04. doi: 10.3390/ijms22031186. PubMed PMID: 33530349; PMCID: PMC7865413.
Li HD, Funk CC, McFarland K, Dammer EB, Allen M, Carrasquillo MM, Levites Y, Chakrabarty P, Burgess JD, Wang X, Dickson D, Seyfried NT, Duong DM, Lah JJ, Younkin SG, Levey AI, Omenn GS, Ertekin-Taner N, Golde TE, Price ND. Integrative functional genomic analysis of intron retention in human and mouse brain with Alzheimer’s disease. Alzheimers Dement. 2021;17(6):984-1004. Epub 2021/01/23. doi: 10.1002/alz.12254. PubMed PMID: 33480174; PMCID: PMC8248162.
Higginbotham L, Ping L, Dammer EB, Duong DM, Zhou M, Gearing M, Hurst C, Glass JD, Factor SA, Johnson ECB, Hajjar I, Lah JJ, Levey AI, Seyfried NT. Integrated proteomics reveals brain-based cerebrospinal fluid biomarkers in asymptomatic and symptomatic Alzheimer’s disease. Sci Adv. 2020 Oct 21;6(43):eaaz9360. doi: 10.1126/sciadv.aaz9360.
Huang M, Modeste E, Dammer E, Merino P, Taylor G, Duong DM, Deng Q, Holler CJ, Gearing M, Dickson D, Seyfried NT, Kukar T. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations. Acta Neuropathol Commun. 2020 Oct 7;8(1):163.
Huang M, Modeste E, Dammer E, Merino P, Taylor G, Duong DM, Deng Q, Holler CJ, Gearing M, Dickson D, Seyfried NT, Kukar T. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations. Acta Neuropathol Commun. 2020 Oct 7;8(1):163. doi: 10.1186/s40478-020-01037-x.
Mangleburg CG, Wu T, Yalamanchili HK, Guo C, Hsieh YC, Duong DM, Dammer EB, De Jager PL, Seyfried NT, Liu Z, Shulman JM. Integrated analysis of the aging brain transcriptome and proteome in tauopathy. Mol Neurodegener. 2020 Sep 29;15(1):56.
Ping L, Kundinger SR, Duong DM, Yin L, Gearing M, Lah JJ, Levey AI, Seyfried NT. Global quantitative analysis of the human brain proteome and phosphoproteome in Alzheimer’s disease. Sci Data. 2020 Sep 28;7(1):315.
Rayaprolu S, Higginbotham L, Bagchi P, Watson CM, Zhang T, Levey AI, Rangaraju S, Seyfried NT. Systems-based proteomics to resolve the biology of Alzheimer’s disease beyond amyloid and tau. Neuropsychopharmacology. 2021;46(1):98-115. Epub 2020/09/09. doi: 10.1038/s41386-020-00840-3. PubMed PMID: 32898852; PMCID: PMC7689445.
Ouellette AR, Neuner SM, Dumitrescu L, Anderson LC, Gatti DM, Mahoney ER, Bubier JA, Churchill G, Peters L, Huentelman MJ, Herskowitz JH, Yang HS, Smith AN, Reitz C, Kunkle BW, White CC, De Jager PL, Schneider JA, Bennett DA, Seyfried NT; Alzheimer’s Disease Genetics Consortium, Chesler EJ, Hadad N, Hohman TJ, Kaczorowski CC. Cross-Species Analyses Identify Dlgap2 as a Regulator of Age-Related Cognitive Decline and Alzheimer’s Dementia. Cell Rep. 2020 Sep 1;32(9):108091.
Beckmann ND, Lin WJ, Wang M, Cohain AT, Charney AW, Wang P, Ma W, Wang YC, Jiang C, Audrain M, Comella PH, Fakira AK, Hariharan SP, Belbin GM, Girdhar K, Levey AI, Seyfried NT, Dammer EB, Duong D, Lah JJ, Haure-Mirande JV, Shackleton B, Fanutza T, Blitzer R, Kenny E, Zhu J, Haroutunian V, Katsel P, Gandy S, Tu Z, Ehrlich ME, Zhang B, Salton SR, Schadt EE. Multiscale causal networks identify VGF as a key regulator of Alzheimer’s disease. Nat Commun. 2020 Aug 7;11(1):3942.
Chang YH, Duong DM, Goll JB, Wood DC, Jensen TL, Yin L, Gelber CE, Seyfried NT, Anderson E, Natrajan MS, Rouphael N, Johnson RA, Sanz P, Mulligan MJ, Hoft DF. Proteomic Analysis of Human Immune Responses to Live-Attenuated Tularemia Vaccine. Vaccines (Basel). 2020 Jul 24;8(3):E413.
Yu L, Tasaki S, Schneider JA, Arfanakis K, Duong DM, Wingo AP, Wingo TS, Kearns N, Thatcher GRJ, Seyfried NT, Levey AI, De Jager PL, Bennett DA. Cortical Proteins Associated with Cognitive Resilience in Community-Dwelling Older Persons. JAMA Psychiatry. 2020 Jul 1:e201807
Swarup V, Chang TS, Duong DM, Dammer EB, Dai J, Lah JJ, Johnson ECB, Seyfried NT, Levey AI, Geschwind DH. Identification of Conserved Proteomic Networks in Neurodegenerative Dementia. Cell Rep. 2020 Jun 23;31(12):107807.
Wingo AP, Fan W, Duong DM, Gerasimov ES, Dammer EB, Liu Y, Harerimana NV, White B, Thambisetty M, Troncoso JC, Kim N, Schneider JA, Hajjar IM, Lah JJ, Bennett DA, Seyfried NT, Levey AI, Wingo TS. Shared proteomic effects of cerebral atherosclerosis and Alzheimer’s disease on the human brain. Nat Neurosci. 2020(6):696-700.
Zhou M, Haque RU, Dammer EB, Duong DM, Ping L, Johnson ECB, Lah JJ, Levey AI, Seyfried NT. Targeted mass spectrometry to quantify brain-derived cerebrospinal fluid biomarkers in Alzheimer’s disease. Clin Proteomics. 2020;17:19.
Koyen AE, Madden MZ, Park D, Minten EV, Kapoor-Vazirani P, Werner E, Pfister NT, Haji-Seyed-Javadi R, Zhang H, Xu J, Deng N, Duong DM, Pecen TJ, Frazier Z, Nagel ZD, Lazaro JB, Mouw KW, Seyfried NT, Moreno CS, Owonikoko TK, Deng X, Yu DS. EZH2 has a non-catalytic and PRC2-independent role in stabilizing DDB2 to promote nucleotide excision repair. Oncogene. 2020 May 26.
Rayaprolu S, Gao T, Xiao H, Ramesha S, Weinstock LD, Shah J, Duong DM, Dammer EB, Webster JA Jr, Lah JJ, Wood LB, Betarbet R, Levey AI, Seyfried NT, Rangaraju S. Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease. Mol Neurodegener. 2020;15:28.
Johnson ECB, Dammer EB, Duong DM, Ping L, Zhou M, Yin L, Higginbotham LA, Guajardo A, White B, Troncoso JC, Thambisetty M, Montine TJ, Lee EB, Trojanowski JQ, Beach TG, Reiman EM, Haroutunian V, Wang M, Schadt E, Zhang B, Dickson DW, Ertekin-Taner N, Golde TE, Petyuk VA, De Jager PL, Bennett DA, Wingo TS, Rangaraju S, Hajjar I, Shulman JM, Lah JJ, Levey AI, Seyfried NT. Large-scale proteomic analysis of Alzheimer’s disease brain and cerebrospinal fluid reveals early changes in energy metabolism associated with microglia and astrocyte activation. Nat Med. 2020 May;26(5):769-780.
Kundinger SR, Bishof I, Dammer EB, Duong DM, Seyfried NT. Middle-Down Proteomics Reveals Dense Sites of Methylation and Phosphorylation in Arginine-Rich RNA-Binding Proteins. J Proteome Res. 2020 Feb 13.
Nguyen TT, Dammer EB, Owino SA, Giddens MM, Madaras NS, Duong DM, Seyfried NT, Hall RA. Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1. J Proteome Res. 2020 Feb 7;19(2):744-755.
Wingo TS, Yang J, Fan W, Min Canon S, Gerasimov ES, Lori A, Logsdon B, Yao B, Seyfried NT, Lah JJ, Levey AI, Boyle PA, Schneider JA, De Jager PL, Bennett DA, Wingo AP. Brain microRNAs associated with late-life depressive symptoms are also associated with cognitive trajectory and dementia. NPJ Genom Med. 2020 Feb 6;5:6.
Sarkar S, Dammer EB, Malovic E, Olsen AL, Raza SA, Gao T, Xiao H, Oliver DL, Duong D, Joers V, Seyfried NT, Huang M, Kukar T, Tansey MG, Kanthasamy AG, Rangaraju S. Molecular Signatures of Neuroinflammation Induced by αSynuclein Aggregates in Microglial Cells. Front Immunol. 2020 Jan 31;11:33.
Gao T, Jernigan J, Raza SA, Dammer EB, Xiao H, Seyfried NT, Levey AI, Rangaraju S. Transcriptional regulation of homeostatic and disease-associated-microglial genes by IRF1, LXRβ, and CEBPα. Glia. 2019 Oct;67(10):1958-1975.
Hsieh YC, Guo C, Yalamanchili HK, Abreha M, Al-Ouran R, Li Y, Dammer EB, Lah JJ, Levey AI, Bennett DA, De Jager PL, Seyfried NT, Liu Z, Shulman JM. Tau-Mediated Disruption of the Spliceosome Triggers Cryptic RNA Splicing and Neurodegeneration in Alzheimer’s Disease. Cell Rep. 2019 Oct 8;29(2):301-316.e10.
Werner E, Alter A, Deng Q, Dammer EB, Wang Y, Yu DS, Duong DM, Seyfried NT, Doetsch PW. Ionizing Radiation induction of cholesterol biosynthesis in Lung tissue. Sci Rep. 2019 Aug 29;9(1):12546.
Higginbotham L, Dammer EB, Duong DM, Modeste E, Montine TJ, Lah JJ, Levey AI, Seyfried NT. Network Analysis of a Membrane-Enriched Brain Proteome across Stages of Alzheimer’s Disease. Proteomes. 2019 Aug 27;7(3). pii: E30.
Zhou M, Duong DM, Johnson ECB, Dai J, Lah JJ, Levey AI, and Seyfried NT. Mass spectrometry-based quantification of Tau in human cerebrospinal fluid using a complementary tryptic peptide standard. J Proteome Res. 2019 Jun 7;18(6):2422-2432.
Gerber KJ, Dammer EB, Duong DM, Deng Q, Dudek SM, Seyfried NT, Hepler JR. Specific Proteomes of Hippocampal Regions CA2 and CA1 Reveal Proteins Linked to the Unique Physiology of Area CA2. J Proteome Res. 2019 Jun 7;18(6):2571-2584.
Wingo AP, Dammer EB, Breen MS, Logsdon BA, Duong DM, Troncosco JC, Thambisetty M, Beach TG, Serrano GE, Reiman EM, Caselli RJ, Lah JJ, Seyfried NT, Levey AI, Wingo TS. Large-scale proteomic analysis of human brain identifies proteins associated with cognitive trajectory in advanced age. Nat Commun. 2019 Apr 8;10(1):1619.
Banerjee A, Phillips BL, Deng Q, Seyfried NT, Pavlath GK, Vest KE, Corbett AH. Proteomic analysis reveals that wildtype and alanine-expanded nuclear poly(A)-binding protein exhibit differential interactions in skeletal muscle. J Biol Chem. 2019 Mar 5.
Gokhale A, Hartwig C, Freeman AAH, Bassell JL, Zlatic SA, Sapp Savas C, Vadlamudi T, Abudulai F, Pham TT, Crocker A, Werner E, Wen Z, Repetto GM, Gogos JA, Claypool SM, Forsyth JK, Bearden CE, Glausier J, Lewis DA, Seyfried NT, Kwong JQ, Faundez V. Systems Analysis of the 22q11.2 Microdeletion Syndrome Converges on a Mitochondrial Interactome Necessary for Synapse Function and Behavior. J Neurosci. 2019 Mar 4.
Swarup V, Hinz FI, Rexach JE, Noguchi KI, Toyoshiba H, Oda A, Hirai K, Sarkar A, Seyfried NT, Cheng C, Haggarty SJ; International Frontotemporal Dementia Genomics Consortium, Grossman M, Van Deerlin VM, Trojanowski JQ, Lah JJ, Levey AI, Kondou S, Geschwind DH. Identification of evolutionarily conserved gene networks mediating neurodegenerative dementia. Nat Med. 2019 Jan;25(1):152-164.
Dai J, Johnson ECB, Dammer EB, Duong DM, Gearing M, Lah JJ, Levey AI, Wingo TS, Seyfried NT. Effects of APOE Genotype on Brain Proteomic Network and Cell Type Changes in Alzheimer’s Disease. Front Mol Neurosci. 2018 Dec 18;11:454.
Srikanth P, Lagomarsino VN, Pearse RV 2nd, Liao M, Ghosh S, Nehme R, Seyfried N, Eggan K, Young-Pearse TL. Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression. Transl Psychiatry. 2018 Nov 8;8(1):245.
Johnson ECB, Dammer EB, Duong DM, Yin L, Thambisetty M, Troncoso JC, Lah JJ, Levey AI, Seyfried NT. Deep proteomic network analysis of Alzheimer’s disease brain reveals alterations in RNA binding proteins and RNA splicing associated with disease. Mol Neurodegener. 2018 Oct 4;13(1):52.
Abreha MH, Dammer EB, Ping L, Zhang T, Duong DM, Gearing M, Lah JJ, Levey AI, Seyfried NT. Quantitative Analysis of the Brain Ubiquitylome in Alzheimer’s Disease. Proteomics. 2018 Sep 19:e1800108.
Wang M, Beckmann ND, Roussos P, Wang E, Zhou X, Wang Q, Ming C, Neff R, Ma W, Fullard JF, Hauberg ME, Bendl J, Peters MA, Logsdon B, Wang P, Mahajan M, Mangravite LM, Dammer EB, Duong DM, Lah JJ, Seyfried NT, Levey AI, Buxbaum JD, Ehrlich M, Gandy S, Katsel P, Haroutunian V, Schadt E, Zhang B. The Mount Sinai cohort of large-scale genomic, transcriptomic and proteomic data in Alzheimer’s disease. Sci Data. 2018 Sep 11;5:180185. doi: 10.1038/sdata.2018.185.
Rangaraju S, Dammer EB, Raza SA, Gao T, Xiao H, Betarbet R, Duong DM, Webster JA, Hales CM, Lah JJ, Levey AI, Seyfried NT. (2018). Quantitative proteomics of acutely-isolated mouse microglia identifies novel immune Alzheimer’s disease-related proteins. Mol Neurodegener. Jun 28;13(1):34.
Bishof I, Dammer EB, Duong DM, Kundinger S, Gearing M, Lah JJ, Levey AI, Seyfried NT. RNA-binding proteins with basic-acidic dipeptide (BAD) domains self-assemble and aggregate in Alzheimer’s disease. (2018) J Biol Chem. 2018 May 25, in press.
Rangaraju S, Dammer EB, Raza SA, Rathakrishnan P, Xiao H, Gao T, Duong DM, Pennington MW, Lah JJ, Seyfried NT, Levey AI. (2018) Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease. Mol Neurodegener. 13(1):24.
Evans PR, Gerber KJ, Dammer EB, Duong DM, Goswami D, Lustberg DJ, Zou J, Yang JJ, Dudek SM, Griffin PR, Seyfried NT, Hepler JR. (2018) Interactome Analysis Reveals Regulator of G Protein Signaling 14 (RGS14) is a Novel Calcium/Calmodulin (Ca(2+)/CaM) and CaM Kinase II (CaMKII) Binding Partner. J Proteome Res. 17(4):1700-1711.
Ping L, Duong DM, Yin L, Gearing M, Lah JJ, Levey AI, Seyfried NT. (2018) Global quantitative analysis of the human brain proteome in Alzheimer’s and Parkinson’s Disease. Sci Data.5:180036.
Rangaraju S, Raza SA, Li NX, Betarbet R, Dammer EB, Duong D, Lah JJ, Seyfried NT, Levey AI. (2018) Differential Phagocytic Properties of CD45(low) Microglia and CD45(high) Brain Mononuclear Phagocytes-Activation and Age-Related Effects. Front Immunol. 9:405.
Chou CC, Zhang Y, Umoh ME, Vaughan SW, Lorenzini I, Liu F, Sayegh M, Donlin-Asp PG, Chen YH, Duong DM, Seyfried N.T., Powers MA, Kukar T, Hales CM, Gearing M, Cairns NJ, Boylan KB, Dickson DW, Rademakers R, Zhang YJ, Petrucelli L, Sattler R, Zarnescu DC, Glass JD, Rossoll W. (2018). TDP-43 pathology disrupts nuclear pore complexes and nucleocytoplasmic transport in ALS/FTD. Nat Neurosci. In press.
Umoh ME, Dammer EB, Dai J, Duong DM, Lah JJ, Levey AI, Gearing M, Glass JD, Seyfried N.T. (2018) A proteomic network approach across the ALS-FTD disease spectrum resolves clinical phenotypes and genetic vulnerability in human brain. EMBO Mol Med. Jan;10(1):48-62.
Bhuripanyo K, Wang Y, Liu X, Zhou L, Liu R, Duong D, Zhao B, Bi Y, Zhou H, Chen G, Seyfried N.T., Chazin WJ, Kiyokawa H, Yin J. (2018) Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer. Sci Adv. 4:e1701393.
Cherry JD, Zeineddin A, Dammer EB, Webster JA, Duong D, Seyfried N.T., Levey AI, Alvarez VE, Huber BR, Stein TD, Kiernan PT, McKee AC, Lah JJ, Hales CM. (2018). Characterization of Detergent Insoluble Proteome in Chronic Traumatic Encephalopathy. J Neuropathol Exp Neurol. 77: 40-49.
Allen M, Wang X, Burgess JD, Watzlawik J, Serie DJ, Younkin CS, Nguyen T, Malphrus KG, Lincoln S, Carrasquillo MM, Ho C, Chakrabarty P, Strickland S, Murray ME, Swarup V, Geschwind DH, Seyfried N.T., Dammer EB, Lah JJ, Levey AI, Golde TE, Funk C, Li H, Price ND, Petersen RC, Graff-Radford NR, Younkin SG, Dickson DW, Crook JR, Asmann YW, Ertekin-Taner N. (2017) Conserved brain myelination networks are altered in Alzheimer’s and other neurodegenerative diseases. Alzheimers Dement. pii: S1552-5260(17)33766-4
McKenzie AT, Moyon S, Wang M, Katsyv I, Song WM, Zhou X, Dammer EB, Duong DM, Aaker J, Zhao Y, Beckmann N, Wang P, Zhu J, Lah JJ, Seyfried N.T., Levey AI, Katsel P, Haroutunian V, Schadt EE, Popko B, Casaccia P, Zhang B. (2017) Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer’s disease. Mol Neurodegener.12(1):82.
Diner I., Nguyen T., and Seyfried N.T. (2017). Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain. Accepted to Journal of Visual Experimentation (JoVE).
An Y, Varma VR, Varma S, Casanova R, Dammer E, Pletnikova O, Chia CW, Egan JM, Ferrucci L, Troncoso J, Levey AI, Lah J, Seyfried N.T., Legido-Quigley C, O’Brien R, Thambisetty M. Evidence for brain glucose dysregulation in Alzheimer’s disease. Alzheimers Dement. 2017 Oct 19. pii: S1552-5260(17)33765-2
Diner I, Dooyema J, Gearing M, Walker LC, Seyfried N.T. Generation of Clickable Pittsburgh Compound B for the Detection and Capture of β-Amyloid in Alzheimer’s Disease Brain. Bioconjug Chem. 28(10):2627-2637.
Rha J., Jones S.K., Fidler J., Banerjee A., Leung S.W., Morris K.J., Wong J.C., Inglis G.A.S., Shapiro L., Deng Q., Cutler A.A., Hanif A.M., Pardue M.T., Schaffer A., Seyfried N.T., Moberg K.H., Bassell G.J., Escayg A., Garcia P.S. and Corbett A.H. (2017). The RNA-binding Protein, ZC3H14, is Required for Proper Poly(A) Tail Length Control, Expression of Synaptic Proteins, and Brain Function in Mice. Hum Mol Genet.
Wingo T.S., Duong D.M., Zhou M., Dammer E.B., Wu H., Cutler D.J., Lah J.J., Levey A.I., and Seyfried N.T. (2017). Integrating next-generation genomic sequencing and mass spectrometry to identify variant peptides in human brain. Accepted at J. Proteome Res.
Zhang Z., Kang S.S., Liu X., Ahn E.H., Zhang Z., He L., Iuvone P.M., Duong D.M., Seyfried N.T., Benskey M.J., Manfredsson F.P., Jin L., Sun Y.E., Wang J.Z. and Ye K. (2017). Asparagine endopeptidase cleaves alpha-synuclein and mediates pathologic activities in Parkinson’s disease. Nat Struct Mol Biol.
Cutler AA, Dammer EB, Doung DM, Seyfried NT, Corbett AH, Pavlath GK. (2017). Biochemical isolation of myonuclei employed to define changes to the myonuclear proteome that occur with aging. Aging Cell. May 23. doi: 10.1111/acel.12604.
Ivanova A.A., Caspary T., Seyfried N.T., Duong D.M., West A.B., Liu Z. and Kahn R.A. (2017). Biochemical characterization of purified mammalian ARL13B indicate that it is an atypical GTPase and ARL3 guanine nucleotide exchange factor (GEF). J Biol Chem
Rangaraju S., Raza S.A., Pennati A., Deng Q., Dammer E.B., Duong D., Pennington M.W., Tansey M.G., Lah J.J., Betarbet R., Seyfried N.T. and Levey A.I. (2017). A systems pharmacology-based approach to identify novel Kv1.3 channel-dependent mechanisms in microglial activation. J Neuroinflammation 14: 128.
Seyfried N.T.*, Dammer E.B., Swarup V., Nandakumar D., Duong D.M., Yin L., Deng Q., Nguyen T., Hales C.M., Wingo T., Glass J., Gearing M., Thambisetty M., Troncoso J.C., Geschwind D.H., Lah J.J. and Levey A.I. (2017). A Multi-network Approach Identifies Protein-Specific Co-expression in Asymptomatic and Symptomatic Alzheimer’s Disease. Cell Syst 4: 60-72 e64. * co-corresponding author and lead contact author.
Baliga N.S., Bjorkegren J.L., Boeke J.D., Boutros M., Crawford N.P., Dudley A.M., Farber C.R., Jones A., Levey A.I., Lusis A.J., Mak H.C., Nadeau J.H., Noyes M.B., Petretto E., Seyfried N.T., Steinmetz L.M. and Vonesch S.C. (2017). The State of Systems Genetics in 2017. Cell Syst 4: 7-15.
Varma V.R., Varma S., An Y., Hohman T.J., Seddighi S., Casanova R., Beri A., Dammer E.B., Seyfried N.T., Pletnikova O., Moghekar A., Wilson M.R., Lah J.J., O’Brien R.J., Levey A.I., Troncoso J.C., Albert M.S. and Thambisetty M. (2017). Alpha-2 macroglobulin in Alzheimer’s disease: a marker of neuronal injury through the RCAN1 pathway. Mol Psychiatry 22: 13-23.
Li Y., Dammer E.B., Zhang-Brotzge X., Chen S., Duong D.M., Seyfried N.T., Kuan C.Y. and Sun Y.Y. (2017). Osteopontin Is a Blood Biomarker for Microglial Activation and Brain Injury in Experimental Hypoxic-Ischemic Encephalopathy. eNeuro 4
Hales C.M., Dammer E.B., Deng Q., Duong D.M., Gearing M., Troncoso J.C., Thambisetty M., Lah J.J., Shulman J.M., Levey A.I. and Seyfried N.T. (2016). Changes in the detergent-insoluble brain proteome linked to amyloid and tau in Alzheimer’s Disease progression. Proteomics 16: 3042-3053.
Soucek S., Zeng Y., Bellur D.L., Bergkessel M., Morris K.J., Deng Q., Duong D., Seyfried N.T., Guthrie C., Staley J.P., Fasken M.B. and Corbett A.H. (2016). The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA. Mol Cell Biol.
Zhang H., Head P.E., Daddacha W., Park S.H., Li X., Pan Y., Madden M.Z., Duong D.M., Xie M., Yu B., Warren M.D., Liu E.A., Dhere V.R., Li C., Pradilla I., Torres M.A., Wang Y., Dynan W.S., Doetsch P.W., Deng X., Seyfried N.T., Gius D. and Yu D.S. (2016). ATRIP Deacetylation by SIRT2 Drives ATR Checkpoint Activation by Promoting Binding to RPA-ssDNA. Cell Rep 14: 1435-1447.
Hu W.T., Watts K.D., Tailor P., Nguyen T.P., Howell J.C., Lee R.C., Seyfried N.T., Gearing M., Hales C.M., Levey A.I., Lah J.J., Lee E.K. and Alzheimer’s Disease Neuro-Imaging I. (2016). CSF complement 3 and factor H are staging biomarkers in Alzheimer’s disease. Acta Neuropathol Commun 4: 14.
Owings J.P., Kuiper E.G., Prezioso S.M., Meisner J., Varga J.J., Zelinskaya N., Dammer E.B., Duong D.M., Seyfried N.T., Alberti S., Conn G.L. and Goldberg J.B. (2016). Pseudomonas aeruginosa EftM Is a Thermoregulated Methyltransferase. J Biol Chem 291: 3280-3290.
Kudelka M.R., Antonopoulos A., Wang Y., Duong D.M., Song X., Seyfried N.T., Dell A., Haslam S.M., Cummings R.D. and Ju T. (2016). Cellular O-Glycome Reporter/Amplification to explore O-glycans of living cells. Nat Methods 13: 81-86.
Zhang Z, Song M, Liu X, Su Kang S, Duong DM, Seyfried NT, Cao X, Cheng L, Sun YE, Ping Yu S, Jia J, Levey AI, Ye K. Delta-secretase cleaves amyloid precursor protein and regulates the pathogenesis in Alzheimer’s disease. Nat Commun. 2015 Nov 9;6:8762. doi: 10.1038/ncomms9762. PubMed PMID: 26549211
Dammer EB, Göttle M, Duong DM, Hanfelt J, Seyfried NT, Jinnah HA. Consequences of impaired purine recycling on the proteome in a cellular model of Lesch-Nyhan disease. Mol Genet Metab. 2015 Apr;114(4):570-9. doi: 10.1016/j.ymgme.2015.02.007. Epub 2015 Mar 5. PubMed PMID: 25769394; PubMed Central PMCID: PMC4390545.
Yang Q, Li W, She H, Dou J, Duong DM, Du Y, Yang SH, Seyfried NT, Fu H, Gao G, Mao Z. Stress induces p38 MAPK-mediated phosphorylation and inhibition of Drosha-dependent cell survival. Mol Cell. 2015 Feb 19;57(4):721-34. doi: 10.1016/j.molcel.2015.01.004. PubMed PMID: 25699712
Jin L, Li D, Alesi GN, Fan J, Kang HB, Lu Z, Boggon TJ, Jin P, Yi H, Wright ER, Duong D, Seyfried NT, Egnatchik R, DeBerardinis RJ, Magliocca KR, He C, Arellano ML, Khoury HJ, Shin DM, Khuri FR, Kang S. Glutamate dehydrogenase 1 signals through antioxidant glutathione peroxidase 1 to regulate redox homeostasis and tumor growth. Cancer Cell. 2015 Feb 9;27(2):257-70. doi: 10.1016/j.ccell.2014.12.006. PubMed PMID: 25670081
Dammer EB, Lee AK, Duong DM, Gearing M, Lah JJ, Levey AI, Seyfried NT. Quantitative phosphoproteomics of Alzheimer’s disease reveals cross-talk between kinases and small heat shock proteins. Proteomics. 2015 Jan;15(2-3):508-19. doi: 10.1002/pmic.201400189. Epub 2014 Dec 17. PubMed PMID: 25332170
Ren RJ, Dammer EB, Wang G, Seyfried NT, Levey AI. Proteomics of protein post-translational modifications implicated in neurodegeneration. Transl Neurodegener. 2014 Oct 30;3(1):23. doi: 10.1186/2047-9158-3-23. eCollection 2014. PubMed PMID: 25671099
Diner I, Hales CM, Bishof I, Rabenold L, Duong DM, Yi H, Laur O, Gearing M, Troncoso J, Thambisetty M, Lah JJ, Levey AI, Seyfried NT. Aggregation properties of the small nuclear ribonucleoprotein U1-70K in Alzheimer disease. J Biol Chem. 2014 Dec 19;289(51):35296-313. doi: 10.1074/jbc.M114.562959. Epub 2014 Oct 29. PubMed PMID: 25355317
Zhang Z, Song M, Liu X, Kang SS, Kwon IS, Duong DM, Seyfried NT, Hu WT, LiuZ, Wang JZ, Cheng L, Sun YE, Yu SP, Levey AI, Ye K. Cleavage of tau by asparagine endopeptidase mediates the neurofibrillary pathology in Alzheimer’s disease. Nat Med. 2014 Nov;20(11):1254-62. doi: 10.1038/nm.3700. Epub 2014 Oct 19. PubMed PMID: 25326800
Ali M, Chernova TA, Newnam GP, Yin L, Shanks J, Karpova TS, Lee A, Laur O, Subramanian S, Kim D, McNally JG, Seyfried NT, Chernoff YO, Wilkinson KD. Stress-dependent proteolytic processing of the actin assembly protein Lsb1 modulates a yeast prion. J Biol Chem. 2014 Oct 3;289(40):27625-39. doi: 10.1074/jbc.M114.582429. Epub 2014 Aug 20. PubMed PMID: 25143386.
Deng Q, Holler CJ, Taylor G, Hudson KF, Watkins W, Gearing M, Ito D, Murray ME, Dickson DW, Seyfried NT, Kukar T. FUS is phosphorylated by DNA-PK and accumulates in the cytoplasm after DNA damage. J Neurosci. 2014 Jun 4;34(23):7802-13. doi: 10.1523/JNEUROSCI.0172-14.2014. PubMed PMID: 24899704
Hales CM, Seyfried NT, Dammer EB, Duong D, Yi H, Gearing M, Troncoso JC, Mufson EJ, Thambisetty M, Levey AI, Lah JJ. U1 small nuclear ribonucleoproteins (snRNPs) aggregate in Alzheimer’s disease due to autosomal dominant genetic mutations and trisomy 21. Mol Neurodegener. 2014 Apr 28;9:15. doi: 10.1186/1750-1326-9-15. PubMed PMID: 24773620
Hales CM, Dammer EB, Diner I, Yi H, Seyfried NT, Gearing M, Glass JD, Montine TJ, Levey AI, Lah JJ. Aggregates of small nuclear ribonucleic acids (snRNAs) in Alzheimer’s disease. Brain Pathol. 2014 Jul;24(4):344-51. doi: 10.1111/bpa.12133. Epub 2014 Apr 14. PubMed PMID: 24571648
Herskowitz JH, Feng Y, Mattheyses AL, Hales CM, Higginbotham LA, Duong DM, Montine TJ, Troncoso JC, Thambisetty M, Seyfried NT, Levey AI, Lah JJ. Pharmacologic inhibition of ROCK2 suppresses amyloid-β production in an Alzheimer’s disease mouse model. J Neurosci. 2013 Dec 4;33(49):19086-98. doi:10.1523/JNEUROSCI.2508-13.2013. PubMed PMID: 24305806.
Hales CM, Rees H, Seyfried NT, Dammer EB, Duong DM, Gearing M, Montine TJ, Troncoso JC, Thambisetty M, Levey AI, Lah JJ, Wingo TS. Abnormal gephyrin immunoreactivity associated with Alzheimer disease pathologic changes. J Neuropathol Exp Neurol. 2013 Nov;72(11):1009-15. doi: 10.1097/01.jnen.0000435847.59828.db. PubMed PMID: 24128675.
Bai B, Hales CM, Chen PC, Gozal Y, Dammer EB, Fritz JJ, Wang X, Xia Q, Duong DM, Street C, Cantero G, Cheng D, Jones DR, Wu Z, Li Y, Diner I, Heilman CJ, Rees HD, Wu H, Lin L, Szulwach KE, Gearing M, Mufson EJ, Bennett DA, Montine TJ, Seyfried NT, Wingo TS, Sun YE, Jin P, Hanfelt J, Willcock DM, Levey A, Lah JJ, Peng J. U1 small nuclear ribonucleoprotein complex and RNA splicing alterations in Alzheimer’s disease. Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16562-7. doi: 10.1073/pnas.1310249110. Epub 2013 Sep 10. PubMed PMID: 24023061
Go YM, Roede JR, Walker DI, Duong DM, Seyfried NT, Orr M, Liang Y, Pennell KD, Jones DP. Selective targeting of the cysteine proteome by thioredoxin and glutathione redox systems. Mol Cell Proteomics. 2013 Nov;12(11):3285-96. doi: 10.1074/mcp.M113.030437. Epub 2013 Aug 14. PubMed PMID: 23946468.
Zhang H, Park SH, Pantazides BG, Karpiuk O, Warren MD, Hardy CW, Duong DM, Park SJ, Kim HS, Vassilopoulos A, Seyfried NT, Johnsen SA, Gius D, Yu DS. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13546-51. doi: 10.1073/pnas.1301463110. Epub 2013 Jul 29. PubMed PMID: 23898190
Wu F, Nicholson AD, Haile WB, Torre E, An J, Chen C, Lee AK, Duong DM, Dammer EB, Seyfried NT, Tong FC, Votaw JR, Yepes M. Tissue-type plasminogen activator mediates neuronal detection and adaptation to metabolic stress. J Cereb Blood Flow Metab. 2013 Nov;33(11):1761-9. doi: 10.1038/jcbfm.2013.124. Epub 2013 Jul24. PubMed PMID: 23881246
Dammer EB, Duong DM, Diner I, Gearing M, Feng Y, Lah JJ, Levey AI, Seyfried NT. Neuron enriched nuclear proteome isolated from human brain. J Proteome Res. 2013 Jul 5;12(7):3193-206. doi: 10.1021/pr400246t. Epub 2013 Jun 17. PubMed PMID: 23768213
Donovan LE, Dammer EB, Duong DM, Hanfelt JJ, Levey AI, Seyfried NT, Lah JJ. Exploring the potential of the platelet membrane proteome as a source of peripheral biomarkers for Alzheimer’s disease. Alzheimers Res Ther. 2013 Jun 13;5(3):32. doi: 10.1186/alzrt186. eCollection 2013. PubMed PMID: 23764030
Kallappagoudar S, Dammer EB, Duong DM, Seyfried NT, Lucchesi JC. Expression, purification and proteomic analysis of recombinant histone H4 acetylated at lysine 16. Proteomics. 2013 May;13(10-11):1687-91. doi: 10.1002/pmic.201300025.Epub 2013 Apr 26. PubMed PMID: 23554095
Herskowitz JH, Gozal YM, Duong DM, Dammer EB, Gearing M, Ye K, Lah JJ, Peng J, Levey AI, Seyfried NT. Asparaginyl endopeptidase cleaves TDP-43 in brain. Proteomics. 2012 Aug;12(15-16):2455-63. doi: 10.1002/pmic.201200006. PubMed PMID:22718532
Wu F, Wu J, Nicholson AD, Echeverry R, Haile WB, Catano M, An J, Lee AK, Duong D, Dammer EB, Seyfried NT, Tong FC, Votaw JR, Medcalf RL, Yepes M. Tissue-type plasminogen activator regulates the neuronal uptake of glucose in the ischemic brain. J Neurosci. 2012 Jul 18;32(29):9848-58. PubMed PMID: 22815500
Dammer EB, Fallini C, Gozal YM, Duong DM, Rossoll W, Xu P, Lah JJ, Levey AI, Peng J, Bassell GJ, Seyfried NT. Coaggregation of RNA-binding proteins in a model of TDP-43 proteinopathy with selective RGG motif methylation and a role for RRM1 ubiquitination. PLoS One. 2012;7(6):e38658. doi: 10.1371/journal.pone.0038658. Epub 2012 Jun 21. PubMed PMID: 22761693
Seyfried NT, Gozal YM, Donovan LE, Herskowitz JH, Dammer EB, Xia Q, Ku L, Chang J, Duong DM, Rees HD, Cooper DS, Glass JD, Gearing M, Tansey MG, Lah JJ, Feng Y, Levey AI, Peng J. Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards. J Proteome Res. 2012 May 4;11(5):2721-38. doi: 10.1021/pr2010814. Epub 2012 Apr 4. PubMed PMID: 22416763
Donovan LE, Higginbotham L, Dammer EB, Gearing M, Rees HD, Xia Q, Duong DM, Seyfried NT, Lah JJ, Levey AI. Analysis of a membrane-enriched proteome from postmortem human brain tissue in Alzheimer’s disease. Proteomics Clin Appl. 2012 Apr;6(3-4):201-11. doi: 10.1002/prca.201100068. PubMed PMID: 22532456
Gozal YM, Seyfried NT, Gearing M, Glass JD, Heilman CJ, Wuu J, Duong DM, Cheng D, Xia Q, Rees HD, Fritz JJ, Cooper DS, Peng J, Levey AI, Lah JJ. Aberrant septin 11 is associated with sporadic frontotemporal lobar degeneration. Mol Neurodegener. 2011 Nov 29;6:82. doi: 10.1186/1750-1326-6-82. PubMed PMID: 22126117
Herskowitz JH, Seyfried NT, Gearing M, Kahn RA, Peng J, Levey AI, Lah JJ. Rho kinase II phosphorylation of the lipoprotein receptor LR11/SORLA alters amyloid-beta production. J Biol Chem. 2011 Feb 25;286(8):6117-27. doi: 10.1074/jbc.M110.167239. Epub 2010 Dec 8. PubMed PMID: 21147781.
Dammer EB, Na CH, Xu P, Seyfried NT, Duong DM, Cheng D, Gearing M, Rees H, Lah JJ, Levey AI, Rush J, Peng J. Polyubiquitin linkage profiles in three models of proteolytic stress suggest the etiology of Alzheimer disease. J Biol Chem. 2011 Mar 25;286(12):10457-65. doi: 10.1074/jbc.M110.149633. Epub 2011 Jan 28. PubMed PMID: 21278249
Herskowitz JH, Seyfried NT, Duong DM, Xia Q, Rees HD, Gearing M, Peng J, Lah JJ, Levey AI. Phosphoproteomic analysis reveals site-specific changes in GFAP and NDRG2 phosphorylation in frontotemporal lobar degeneration. J Proteome Res. 2010 Dec 3;9(12):6368-79. doi: 10.1021/pr100666c. Epub 2010 Oct 22. PubMed PMID: 20886841; PubMed Central PMCID: PMC2997170.
Franco M, Seyfried NT, Brand AH, Peng J, Mayor U. A novel strategy to isolate ubiquitin conjugates reveals wide role for ubiquitination during neural development. Mol Cell Proteomics. 2011 May;10(5):M110.002188. doi: 10.1074/mcp.M110.002188. Epub 2010 Sep 22. PubMed PMID: 20861518.
Seyfried NT, Gozal YM, Dammer EB, Xia Q, Duong DM, Cheng D, Lah JJ, Levey AI, Peng J. Multiplex SILAC analysis of a cellular TDP-43 proteinopathy model reveals protein inclusions associated with SUMOylation and diverse polyubiquitin chains. Mol Cell Proteomics. 2010 Apr;9(4):705-18. doi: 10.1074/mcp.M800390-MCP200. Epub 2010 Jan 4. PubMed PMID: 20047951.
2009
Xu P, Duong DM, Seyfried NT, Cheng D, Xie Y, Robert J, Rush J, Hochstrasser M, Finley D, Peng J. Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation. Cell. 2009 Apr 3;137(1):133-45. doi: 10.1016/j.cell.2009.01.041. PubMed PMID: 19345192.
2008
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J. Systematic approach for validating the ubiquitinated proteome. Anal Chem. 2008 Jun 1;80(11):4161-9. doi: 10.1021/ac702516a. Epub 2008 Apr 24. PubMed PMID: 18433149
Seyfried NT, Huysentruyt LC, Atwood JA 3rd, Xia Q, Seyfried TN, Orlando R. Up-regulation of NG2 proteoglycan and interferon-induced transmembrane proteins 1 and 3 in mouse astrocytoma: a membrane proteomics approach. Cancer Lett. 2008 May 18;263(2):243-52. doi: 10.1016/j.canlet.2008.01.007. Epub 2008 Feb 20. PubMed PMID: 18281150; PubMed Central PMCID: PMC2726046.
Baker DL, Seyfried NT, Li H, Orlando R, Terns RM, Terns MP. Determination of protein-RNA interaction sites in the Cbf5-H/ACA guide RNA complex by mass spectrometric protein footprinting. Biochemistry. 2008 Feb 12;47(6):1500-10. doi: 10.1021/bi701606m. Epub 2008 Jan 19. PubMed PMID: 18205399.
2007
Seyfried NT, Atwood JA 3rd, Yongye A, Almond A, Day AJ, Orlando R, Woods RJ. Fourier transform mass spectrometry to monitor hyaluronan-protein interactions: use of hydrogen/deuterium amide exchange. Rapid Commun Mass Spectrom. 2007;21(2):121-31. PubMed PMID: 17154353
2006
Kuznetsova SA, Issa P, Perruccio EM, Zeng B, Sipes JM, Ward Y, Seyfried NT, Fielder HL, Day AJ, Wight TN, Roberts DD. Versican-thrombospondin-1 binding in vitro and colocalization in microfibrils induced by inflammation on vascular smooth muscle cells. J Cell Sci. 2006 Nov 1;119(Pt 21):4499-509. Epub 2006 Oct 17. PubMed PMID: 17046999.
2005
Seyfried NT, Day AJ, Almond A. Experimental evidence for all-or-none cooperative interactions between the G1-domain of versican and multivalent hyaluronan oligosaccharides. Matrix Biol. 2006 Jan;25(1):14-9. Epub 2005 Sep 26. PubMed PMID: 16185858.
Seyfried NT, Blundell CD, Day AJ, Almond A. Preparation and application of biologically active fluorescent hyaluronan oligosaccharides. Glycobiology. 2005 Mar;15(3):303-12. Epub 2004 Oct 20. PubMed PMID: 15496500.
Joki T, Machluf M, Atala A, Zhu J, Seyfried NT, Dunn IF, Abe T, Carroll RS, Black PM. Continuous release of endostatin from microencapsulated engineered cells for tumor therapy. Nat Biotechnol. 2001 Jan;19(1):35-9. PubMed PMID: 11135549.
Joki T, Heese O, Nikas DC, Bello L, Zhang J, Kraeft SK, Seyfried NT, Abe T, Chen LB, Carroll RS, Black PM. Expression of cyclooxygenase 2 (COX-2) in human glioma and in vitro inhibition by a specific COX-2 inhibitor, NS-398. Cancer Res. 2000 Sep 1;60(17):4926-31. PubMed PMID: 10987308.